Vitamin D Measurement: Clinical Practice and Research Implications

Published:November 24, 2022DOI:


      • Emerging evidence identifies vitamin D’s role in extraskeletal health conditions.
      • Evidence-based vitamin D supplementation recommendations remain controversial.
      • Vitamin D laboratory assays are variable and not universally standardized.
      • Lack of assay standardization attenuates comparison and pooling of results.
      • Vitamin D measurement challenges impact nurses’ clinical and research decisions.


      Vitamin D is a complex vitamin that influences physiology and pathophysiology across the lifespan. Vitamin D research has increased exponentially in the last decade, influencing discussions about its measurement and appropriateness of the current clinical guidelines. This report summarizes the challenges related to vitamin D measurement, the impact of those challenges on clinical guidelines, and the implications for nurse practitioners providing clinical care and conducting research. Although the status of vitamin D measurement and clinical recommendations is still unresolved, nurse practitioners and researchers require an awareness of the current state of the science to make important clinical and research decisions.


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      Stephanie Binick, DNP, FNP-BC, is a NP at University of Washington Medical Center, Seattle, and can be contacted at [email protected] .


      Sarah W. Matthews, DNP, FNP-BC, is an affiliate assistant professor, Kendra J.


      Kamp, PhD, RN is an assistant professor, and Margaret Heitkemper, PhD, RN, is a professor and chairperson all at the School of Nursing, University of Washington, Seattle